ABSTRACT
The present study was conducted to correlate the biotypes of Gardnerella vaginalis strains isolated from cases of bacterial vaginosis and their virulence factors. Thirty-two strains of G. vaginalis isolated from cases of bacterial vaginosis were biotyped. Adherence to vaginal epithelial cells, biofilm production, surface hydrophobicity, phospholipase C and protease activity were tested on these isolates. Biotype 1 was the most prevalent (8; 25%), followed by biotype 2 (7; 21.9%) and biotypes 5 and 8 (5; 15.6%). We did not find any statistical correlation between G. vaginalis biotypes and its virulence factors. Virulence factors expressed by G. vaginalis were not associated with a single biotype.
Subject(s)
Adult , Bacterial Adhesion , Bacterial Typing Techniques , Biofilms/growth & development , Epithelial Cells/microbiology , Female , Gardnerella vaginalis/chemistry , Gardnerella vaginalis/classification , Gardnerella vaginalis/isolation & purification , Gardnerella vaginalis/pathogenicity , Humans , Hydrophobic and Hydrophilic Interactions , Peptide Hydrolases/metabolism , Type C Phospholipases/metabolism , Vaginosis, Bacterial/microbiology , Virulence Factors/geneticsABSTRACT
The etiology of rotavirus in acute diarrhoeal illness in children 0-5 years of age, admitted to the Pediatric wards of Kasturba Medical College Hospital, Manipal was studied, over a period of one year. Rotavirus in the faecal samples detected by the slide latex agglutination test accounted for 14.9% of the diarrheas with maximum incidence in the 7-12 months of age group (57.5%). Bacterial enteropathogens continued to play a significant role in diarrheal diseases. Salmonella enteritis was found more in the age group 0-6 months and shigellosis in 37-60 months. In a control study of 100 children who had no diarrhea, 2 were found positive for rotaviruses.
Subject(s)
Acute Disease , Anti-Bacterial Agents/classification , Candida/drug effects , Child , Child, Preschool , Diarrhea/drug therapy , Drug Resistance, Microbial , Enterovirus/drug effects , Feces/microbiology , Female , Humans , Infant , Infant, Newborn , Male , Rotavirus/drug effects , Salmonella typhimurium/drug effectsABSTRACT
Fungi were isolated from 67 cases out of the 295 cases of corneal ulcers investigated. Aspergillus species and species of Candida were the major fungal members isolated. Allescheria boydii was isolated from 3 cases, having no previous history of injury to the eye or infection with bacterial or viral agents. A boydii corneal infection is a rare occurrence. Higher incidence of mystic keratitis was seen among females than males. No relationship to seasonal changes could be established. Bacterial infection was associated in 46. 27% of the cases of mycotic keratitis and Staphylococcus was the predominant bacterial pathogen observed.